• Understand methodologies and molecular strategies for creating animal and plant genetic models.
• Explore applications and strategies for using genetic models in basic, biomedical, and agrifood research.
• Gain insight into site-directed mutagenesis, gene targeting, and recent in vivo gene transfer techniques as essential tools for studying gene function and expression control.
Content
Lectures: I. Animal Genetic Models • Animal Transgenesis: o Additive transgenesis in mice and livestock. o Subtractive and substitutive transgenesis via homologous recombination. o Genome modification using CRISPR. o Applications and strategies (e.g., gene regulation and function in mammals, creation of biomedical model animals, production of recombinant proteins). • Animal Models of Human Pathologies. II. Other Genetic Models • Why use other genetic models? • Genetic screens by mutagenesis in Arabidopsis thaliana. • Developmental genetics in Danio rerio (zebrafish) and Drosophila melanogaster. • Deciphering protein interaction networks in Saccharomyces cerevisiae. Tutorials: • Scientific analysis within the thematic area: rigorous interpretation, critical analysis, mastery of specialized vocabulary; presentation and discussion of a scientific problem with peers.
Additional Information
• Understand methodologies and molecular strategies for creating animal and plant genetic models.
• Explore applications and strategies for using genetic models in basic, biomedical, and agrifood research.
• Gain insight into site-directed mutagenesis, gene targeting, and recent in vivo gene transfer techniques as essential tools for studying gene function and expression control.
